Ambiscript is a ambigraphic nucleic acid notation, which uses character symmetries to facilitate DNA complementation and the identification of palindromes. The notation was first described in 2008 and is further detailed here under the Original tab. More recently we’ve adapted the script for the visual display of nuclear polymorphisms in multiple sequence alignments. The application for rendering these color-coded alignments is known as Ambiscript Mosaic and is available for online use or download under the Mosaic tab.